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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: Dev Biol. 2014 Oct 18;396(2):224–236. doi: 10.1016/j.ydbio.2014.10.005

Figure 4. Expression pattern of growth plate transcripts by mRNA in situ hybridization.

Figure 4

Representative expression analyses of Col2a1 (A–C), Sox9 (D–F), Col10a1 (G–I), Ihh (J–L), Ptch1 (M–O), and Fgfr3 (P–R) transcripts in 18.5 dpc tibia sections from wild-type, mutant, and rescue embryos. The panels demonstrating Ihh data contain insets that enlarge the prehypertrophic/hypertrophic zone of expression. Results were observed consistently in five independent experiments with at least four embryos for each mRNA probed. In situ hybridizations with DIG-labeled RNA probes were performed on 40 µm sections; cartilage elements are outlined for clarity.