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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: Dev Biol. 2014 Oct 18;396(2):224–236. doi: 10.1016/j.ydbio.2014.10.005

Figure 6. Proliferation in mutant and rescue embryonic growth plates.

Figure 6

Embryonic femurs 18.5 dpc stained with DAPI and anti-phospho-histone H3 (PHH3) for analysis of mitotic chondrocytes within the proliferative zone. (A–C) DAPI-stained proliferative chondrocytes demonstrate partial columnar formation in rescue embryos (red arrow heads) compared to mutants. (A’’–C’’) Merged images show that rescue proliferative chondrocytes include double the percentage of pHH3-staining cells in the growth plate compared to mutants, which is comparable to wild-type levels. (D) Quantification of pHH3-positive proliferative chondrocytes in the growth plate. n=3–6 embryos/genotype, *p<0.001 vs. +/+, #p<0.01 vs. cmdBc/cmdBc, one-way ANOVA with Tukey’s post-hoc testing.