(A) UGS mesenchyme was isolated from 14 dpc and 17 dpc male UGS. Ar DNA methylation assessed by methylated DNA immunoprecipitation and normalized to IgG control. (B,b) 14 dpc and (C,c) 17 dpc lower urinary tract sagittal sections were stained by immunohistochemistry to visualize AR (red) E-cadherin protein (CDH1, green). Cell nuclei were stained with DAPI. Insets represent magnified images. Abbreviations: E, epithelium; M, mesenchyme. Results are mean ± SE, n=5/group. Asterisk indicates significant difference from control p≤0.05.