Figure 3. Expression of Nwk2 suppresses excess dendrite number in Sp4 knockdown neurons.

CGNs were transfected with plasmids expressing GFP together with shRNAs targeting Sp4, Nwk2 or a control (Ctrl) and, where indicated, an expression vector encoding Nwk2. 6 days post-transfection, the neurons were immunostained with anti-GFP. (A) Images of representative transfected neurons. Arrowhead indicates axon. Scale bar is 10μm. (B) Quantification of primary dendrite numbers. Values represent mean ± SEM of an experiment repeated 3 times and analysed by ANOVA followed by a post-hoc Tukey test;*P<0.05, n.s.= not significant.