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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: Dev Biol. 2014 Oct 14;396(2):183–200. doi: 10.1016/j.ydbio.2014.08.038

Figure 6. Renal clearance and PCT endocytosis are abrogated in prkcι/ζ deficient embryos.

Figure 6

Embryos were injected with 40-kDa dextran-FITC at 48 hpf, and imaged at 72, 84, and 96 hpf. Control wild-type embryos displayed renal clearance by diminution of the net fluorescent signal intensity over time, as well as dextran-FITC internalization throughout the PCT (white arrows) during its progressive morphogenesis. Double prkcι/ζ morphants displayed severe fluid accumulation, notably pericardial edema that remained strongly positive for the dextran-FITC conjugate label, and the PCT was not labeled by dextran endocytosis (Left panels, lateral views; right panels, dorsal views, with exception of bottom right which shows a lateral/dorsal angled view).