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. 2014 Dec;184(12):3170–3175. doi: 10.1016/j.ajpath.2014.08.024

Figure 4.

Figure 4

The M. tuberculosis bacilli are preferentially recovered from CD271+CD45− bone marrow cells after anti-tuberculosis therapy. Mice were infected with 72 ± 21 M. tuberculosis Erdman bacilli by aerosol. Starting at day 28 of M. tuberculosis infection, mice received rifampicin and isoniazid in the drinking water. After 4 weeks of antibiotic therapy, BM was harvested, followed by separation of CD271+CD45− and CD271−CD45+ cell fractions by negative selection. Cells were dispersed for M. tuberculosis culture, and CFU was determined after at least 3 weeks of incubation at 37°C. Data are from four to six mice per group and shown as the means ± SEM. Control colony forming unit (CFU) yields for mice receiving sterile water (instead of antibiotics) are depicted in Figure 2 (experiments were performed concomitantly).