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. Author manuscript; available in PMC: 2014 Dec 10.
Published in final edited form as: Vet Immunol Immunopathol. 2011 Jun 12;143(0):255–264. doi: 10.1016/j.vetimm.2011.06.020

Figure 2.

Figure 2

Feline tetherin restricts FIV and HIV-1 particle release and is not overcome by the HIV-1 accessory protein Vpu. (A) 293T cells were co-transfected with the FIV expression plasmids FP93 (Gagpol), pGinSin (GFP), pMDG (VSV-G) and indicated amounts of feline tetherin (feTHN) plasmid DNA. Infectious virus yield (expressed as percentage of infection) was determined by transducing CrFK cells with the pseudotype-containing culture supernatants of the producer cells and by quantifying the percentage of GFP-expressing cells using flow cytometry (± s.d., n=3). (B) 293T cells were co-transfected with the HIV-1 ΔVpu expression plasmids p8.91 (Gagpol), CSGW (GFP) and pMDG and indicated amounts of feTHN plasmid DNA. The infectious virus yield was determined as described for (A). (C) 293T cells were co-transfected with the HIV-1 wild-type expression plasmids p8.2 (Gagpol), CSGW (GFP) and pMDG and indicated amounts of feTHN plasmid DNA. The infectious virus yield was determined as described for (A). (D) Western blot analysis (anti-p24 capsid) of 293T cell lysates and virions after co-transfection of FIV, HIV-1 ΔVpu or HIV-1 wild-type expression plasmids and varying amounts of feTHN plasmid DNA.