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. 2014 Dec 10;4:7395. doi: 10.1038/srep07395

Figure 2. Effect of IFN-β on DENV and JEV focus formation in HeLa cells.

Figure 2

HeLa cells were infected with DENV2 strain 16681 or JEV strain JaOArS982. At 12 h p.i., the culture medium was replaced with 0 to 500 U/ml of the human recombinant-IFN-β1a in MEM. At 4 days p.i., foci were detected by immunostaining (A) and the vRNA in the culture fluid was quantified by RT-qPCR (B). The HeLa cells were treated with anti-IFN cocktail consisting of 2,000 neutralization U/ml of the anti-human IFN-β Ab and 20 μg/ml of the anti-human IFN-α/βR2 (CD118) 1 h before the viral infection. The concentration of the anti-IFN cocktail was maintained after the viral infection. At 4 days p.i. the foci were detected by immunostaining (C), and the vRNA in the culture fluid was quantified by RT-qPCR (D). The mean ± SE of the vRNA copy number was obtained from two independent duplicate experiments. The values between two groups were tested by Welch test analysis. The asterisks indicate a statistical significance at p < 0.01, and ND indicates no significant difference.