Figure 6.

A thought experiment illustration shows that in both the high-diffusion extreme (a) and the low-diffusion extreme (b), the resulting feature is broad. (c) Graph shows the relationship between the diffusion coefficients of reagents and the final full-width half-max size of the generated feature in the case with a gel of 600 µm in width. The same relationship is shown in (d) for a gel 150 µm in width. It shows that there is an optimal diffusion coefficient for a given size gel which produces a feature of approximately 10% of the full width of the gel.