Figure 5.

Enzyme loading as a function of porosity for SH-microspheres prepared with varying formulation parameters. Triangles represent varying theoretical base content microspheres (differing amounts of MgCO₃ in the organic phase: 0%, 1.5%, 4.3%, and 11.0%,), circles represent varying inner water phase volume microspheres (differing volumes of inner water phase solution (trehalose in PBS, pH 7.4): 25 μl, 100 μl, 200 μl, and 350 μl), and squares represent varying polymer concentration microspheres (differening amounts of PLGA (50:50, i.v. = 0.57 dL/g) in 1 ml CH₂Cl₂: 200 mg, 260 mg, 320 mg, and 400 mg).