Table 1.
Effect of formation variables on self-healing microsphere propertiesa
| Method of adjusting porosity in Formulation | Variable in formulation | Porosity of self-healing microspheres (%) | 28-day lysozyme release (%) | Monomeric enzyme remaining (%) | Insoluble enzyme remaining (%) | Mass balance (%)b |
|---|---|---|---|---|---|---|
| Varying Base Content | 0% MgCO3 | 60.0 | 30.4 ± 0.7 | 57 ± 2 | 4 ± 1 | 92 ± 2 |
| 1.5% MgCO3 | 64.3 | 41.3 ± 0.5 | 47 ± 1 | 3 ± 1 | 91 ± 1 | |
| 4.3% MgCO3 | 68.8 | 51 ± 4 | 46 ± 1 | 3 ± 1 | 99 ± 4 | |
| 11% MgCO3 | 66.2 | 57 ± 4 | 51 ± 1 | 1.4 ± 0.1 | 110 ± 4 | |
| Varying Inner Water Phase Volume | 25 μL | 49.5 | 30 ± 3 | 76 ± 4 | 11 ± 2 | 117 ± 5 |
| 100 μL | 60.6 | 25.9 ± 0.3 | 64 ± 3 | 6 ± 1 | 96 ± 3 | |
| 200 μL | 64.0 | 33 ± 2 | 62 ± 2 | 5 ± 1 | 100 ± 3 | |
| 350 μL | 59.7 | 24.5 ± 0.6 | 55 ± 1 | 5 ± 1 | 85 ± 1 | |
| Varying Polymer Concentration | 200 mg/mL | 65.2 | 34.4 ± 0.3 | 55 ± 2 | 4 ± 1 | 93 ± 2 |
| 260 mg/mL | 61.0 | 20.6 ± 0.8 | 74 ± 2 | 6 ± 1 | 100 ± 2 | |
| 320 mg/mL | 72.7 | 46.6 ± 0.5 | 42 ± 2 | 6 ± 3 | 95 ± 3 | |
| 400 mg/mL | 64.6 | 57 ± 2 | 30 ± 2 | 3 ± 1 | 89 ± 2 |
a
All data reported as mean ± SEM (n=3); formulation conditions listed in Experimental Methods;
b
Mass balance = 28-day release + monomeric remaining + insoluble remaining; no soluble aggregate in residual samples.