Fig 2.

The cooperative effect of HBx and RMP in the antiapoptosis of HCC cells. (A). HepG2 cells were transfected with expression vectors as indicated. Two days later, cells were harvested and subjected to apoptosis analysis by flow cytometry. The percentage of apoptotic cells were scored and depicted graphically in (C, left panel). (B). HepG2 cells were transfected with expression vectors as indicated. One day later, cells were irradiated at 6 Gy and cultured for additional 2 days. Finally cells were harvested and subjected to apoptosis analysis by flow cytometry. The percentage of apoptotic cells were scored and depicted graphically in (C, right panel). Student's t test, *, p<0.05; **, p<0.01; ***, p<0.001, relative to controls.