Fig 5.

HBx does not regulate the expression of RMP in HCC cells. (A). HepG2 cells were transfected with increasing amounts of HBx expression vector as indicated. qRT-PCR was performed for the quantative determination of the expression of HBx (left panel) and endogenous RMP (right panel). (B). HepG2 cells were transfected with increasing amounts of HBx expression vector as indicated. Cell lysates were extracted for the Western blot analysis (left panel) with antibodies against HBx, RMP or GAPDH as indicated. The protein expression of HBx (middle panel) and RMP (right panel) was quantitated against GAPDH. Results are reported as means ±S.D. of three independent experiments.