Fig 6.

HBx interacts with RMP in HCC cells. (A). HepG2 cells were cotransfected with HBx and Flag-RMP expression vectors. Cells were fixed and immunostained with antibodies against RMP conjugated with Cy3 and HBx antibody conjugated with FITC. The stained cells were analyzed and photographed under a confocal laser scanning microscope. (B). Flag-RMP expression vectors were cotransfected into HepG2 cells stably expressing HBx. Cell lysates were extracted and immunoprecipitation was performed with antibody against Flag. The immunoprecipitates were fractionated in 12.5% SDS-PAGE and subjected to Western blot analysis with antibodies against HBx and RMP as indicated. Preimmune IgG was applied as control of immunoprecipiation (Ctr). (C). Lysates were extracted from HepG2 cells stably expressing HBx. Immunoprecipitation was performed with antibody against HBx or with the preimmue IgG as a control (Ctr). The immunoprecipitates were fractionated in 12.5% SDS-PAGE and subjected to Western blot analysis with antibodies against HBx and RMP as indicated.