Fig 7.

HBx and RMP collaborately regulate apoptotic genes. (A). HepG2 cells (lane 1) were transfected with empty vector (lane 2), expression vectors for RMP (lane 3), HBx (lane 4), or both RMP and HBx (lane 5). Protein was extracted and Western blot analysis was carried out with antibodies against Bax, Bcl-2 and GAPDH which was applied for the normalization. The relative expression of Bax and Bcl-2 against GAPDH were graphically depicted in (B). (C). HepG2 cells were transfected with vectors as indicated. The mRNA was extracted and quantative RT-PCR was carried out for the determination of expression of p53. The expression of GAPDH was also determined for the normalization of expression of above factors. Student's t test, *, p<0.05; **, p<0.01, relative to controls.