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. 2014 Jul 1;2:14011. doi: 10.1038/boneres.2014.11

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Copyright © 2014 Sichuan University

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Equimolar expression of human hnRNPC1 and C2 confers resistance to 1,25(OH)₂D in mouse MC3T3-E1 osteoblasts. (a) Schematic representation of the mouse col1a1 2.3 kB promoter-driven piconoviral P2A sequence construct for the co-expression of human (hu) hnRNPC1 and C2 proteins with a GFP reporter. (b) Western blot analyses showing overexpression of hnRNPC1 and C2 proteins using the P2A construct enriched in the nuclear fraction of MC3T3-E1 cells at 96 h post transfection. Empty vector (E), and high (H, 2 μg per well) and low (L, 0.1 μg per well) levels of P2A expression vector cDNA were transfected into MC3T3 cells. (c) 1,25(OH)₂D (0.1–10 nmol⋅L⁻¹; 6 h) induction of mouse osteoblastic genes after transfection of empty vector or hnRNPC1/C2 P2A expression construct in MC3T3-E1 96 h post transfection.