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. 2014 Aug 22;15(1):283. doi: 10.1186/1471-2105-15-283

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© Jacobs et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of 95 % confidence intervals on the target concentration for different estimation procedures. The analysis per replicate shows typical 95% confidence intervals for single samples. Each replicate presents a random sample with expected concentration λ=1.25 target copies per partition and 5% pipette error. Option 1 on the left shows the 95% confidence interval calculated with a single sample method pooling the partitions of the 8 technical replicates before estimating the concentration and its variance with Poisson statistics. Option 2 on the right uses a replicate based method to estimate a 95% confidence interval based on the 8 individual replicates. Both the concentration and its variance were calculated using the empirical mean and variance of the concentration estimates of 8 independent replicates.