Fig. 1. Irf7 5’-UTR analyzed on a denaturing polyacrylamide gel. pBluescript II SK(+)Irf7 5’-UTR1-215 was linearized using BamH1. Transcripts of the Irf7 5’-UTR1-215 were created using an in vitro transcript assay. (A) The newly generated transcripts appeared as two bands on a 2% agarose gel, even though we denatured them at high temperature, indicating the presence of some secondary structure. (B) A cDNA of the transcript was synthesized using a [γ-32P]ATP-labeled probe and analyzed on a 15% denaturing urea polyacrylamide-bisacrylamide gel. A cDNA of the exact size was clearly seen as a single band in comparison with the pBluescript II SK (+) Irf7 5’-UTR1-155 control cDNA. *SM: size marker.
