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. 2014 Sep 8;7:430. doi: 10.1186/1756-3305-7-430

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© Garcia et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phylogenetic analysis of the salivary thyropin proteins from different tick species. Phylogram resulting from the alignment of 11 full-length protein sequences from the sialotranscriptomes of A. triste (At-7216, At-2612, At-24288, At-24290 and At-24291), A. parvum (Ap-10275, Ap-10276 and Ap-10274) and A. cajennense (Ac-103065, Ac-50867 and Ac-36285) as well as sequences from other ticks previously deposited in the NCBI protein database, including A. maculatum (Am-1991, Am-8397, Am-5097 and Am-4869), A. variegatum (Av-3222), Ixodes ricinus (Ir-1197, Ir-0875, Ir-1199 and Ir-1195), Rhipicephalus pulchellu s (Rp-0373, Rp-8209, Rp-0427 and Rp-8045), R. sanguineus (Rs-8564) and Ornithodoros moubata (Om-2539). The database access numbers used in this analysis are listed in Additional file 5: AF5. The neighbor-joining method with 1,000 bootstrap replicates was used for analysis in MEGA 5.05 software. The numbers on the nodes indicate the bootstrap support. Brackets highlight branches with more than 72% bootstrap support. The bar at bottom indicates 10% amino acid divergence.