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. 2014 Sep 27;14:520. doi: 10.1186/1471-2334-14-520

Figure 2.

Figure 2

The RXR antagonist HX531 reduced the viability of L3/L4 T. muris in vitro . Worms were extracted from mice at day 24 post-infection and incubated for 24 hours with vehicle, the anthelmintic mebendazole, the RXR agonist HX630, the RXR antagonist HX531 or bleach. Worm viability was assessed using (A) a motility score, in which 0 = dead, 1 = very low motility, 2 = low motility and 3 = normal motility, or (B) MTT assay. An Andor camera was used to capture the movement of T. muris by filming for 20s. Pixel changes from frame to frame were captured as an indicator or motility (C) and quantified using Matlab software (D). Data are representative of two independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, compared to controls, unless denoted otherwise.