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. 2014 Nov 26;12:326. doi: 10.1186/s12967-014-0326-7

Figure 2.

Figure 2

Effects of berberine and its derivatives on activation of MAPK signaling pathways induced by oxLDL. (A-B) Cells were pretreated with berberine for 1 h, and then incubated with oxLDL for 10, 30 and 60 min. The cell lysates were collected for Western blotting to measure protein levels of phospho (p)-p38, p-ERK1/2, and p-JNK. (A) Representative bands of Western blotting. (B) Quantification of p-p38, p-ERK1/2 and p-JNK bands. (C-D)Cells were pretreated with berberine or dhBBR, or Di-MeBBR for 1 h, followed by incubation with oxLDL for 10, 30 and 60 min. The cell lysates were collected for Western blotting to measure phospho (p)-p38 and p-JNK. (C) Representative bands of Western blotting. (D) Quantification of p-p38, p-ERK1/2 and p-JNK bands.GAPDH expression was used for protein level normalization. *P < 0.05 vs. BBR group.