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. Author manuscript; available in PMC: 2014 Dec 10.
Published in final edited form as: J Proteome Res. 2010 Oct 1;9(10):5445–5460. doi: 10.1021/pr100678k

Figure 1.

Figure 1

SILAC workflow. A combined strategy involving RNAi against Cdc7, SDS-PAGE protein fractionation, LC–MS/MS data acquisition with a LTQ-Orbitrap (Thermo Fisher Scientific), and downstream bioinformatics for identification, quantification, and functional annotation was applied in this study.