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Western blotting of a cyclic permuted triplicate preparation (see Material and Methods) of a cellular total lysate (T) and of nuclear (N), mitochondrial (M) and cytoplasmic (C) subcellular preparations. Sample loadings and dilutions of primary and secondary antibodies were adjusted to verify presence/absence of the proteins in the different fractions, that is, relative spot intensities between different samples are not indicative of relative abundance in different fractions.
