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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Neurol. 2014 Oct 22;263:132–140. doi: 10.1016/j.expneurol.2014.10.007

Figure 3.

Figure 3

The influence of hypoxic dose on the expression of angiogenic and tight junction proteins on cerebral vessels. Dual-IF was performed on frozen sections of the frontal lobe from mice exposed to different hypoxic levels (8-16% O2) or normoxia for 7 days using antibodies specific for CD31 or fibronectin, CD31 or α5 integrin, or claudin-5. Scale bar = 100μm. Quantification of the changes in protein expression level are displayed in graphs. All experiments were performed with four different animals per condition, and the results expressed as the mean ± SEM of the % change compared to normoxic conditions. Note that increases in vascular fibronectin and α5 integrin expression were not observed until hypoxic levels reached 12% O2 or lower. In contrast, claudin-5 expression levels were significantly increased at the milder hypoxic level of 14% O2. * P < 0.05, ** P < 0.01.