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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Neurol. 2014 Oct 22;263:132–140. doi: 10.1016/j.expneurol.2014.10.007

Figure 5.

Figure 5

Endothelial proliferation is not directly linked to an increased number of large area vessels. A-B. Hypoxic-induced endothelial proliferation is restricted to capillaries. Dual-IF was performed on frozen sections of the frontal lobe from mice exposed to 8% O2 hypoxia for 7 days using antibodies specific for CD31 or Ki67 (A) or α-SMA or Ki67 (B). Scale bar = 50μm. Note that proliferating endothelial cells were located within small diameter capillaries, but they were never found within larger diameter vessels (A) or α-SMA-positive arterioles (B). C. The influence of different levels of hypoxia on vessel size distribution following vascular remodeling. Frozen sections of frontal lobe taken from mice exposed to different hypoxic levels (8-16% O2) or normoxia for 7 days were immunostained for CD31, photographed and vessel size distribution analysis performed using Volocity software. All points represent the mean ± SEM of four subjects. Note that 13% O2 marginally increased the number of small area vessels, but did not increase the number of large area (> 500μm2) vessels. In contrast, the slightly more hypoxic level of 12% O2 significantly increased the number of large area (> 500μm2) vessels. * P < 0.05, ** P < 0.01.