Table 1.
Media and stock solutions used for morphological characterisation.
| Czapek stock solution (100 ml) (Pitt 1979) | |
| NaNO3 NaNO3 | 30 g |
| KCl | 5 g |
| MgSO4·7H2OMgSO4·7H2O | 5 g |
| FeSO4·7H2OFeSO4·7H2O | 0.1 g |
| dH2OdH2O | 100 ml |
| *Store at 4–10 °C | |
| Trace elements stock solution (100 ml) | |
| CuSO4·5H2OCuSO4·5H2O | 0.5 g |
| ZnSO4·7H2OZnSO4·7H2O | 0.1 g |
| dH2OdH2O | 100 ml |
| *Store at 4–10 °C | |
| Blakeslee's Malt extract agar (MEAbl,Blakeslee 1915) | |
| Malt extract (Oxoid) | 20 g |
| Peptone (Oxoid) | 1 g |
| Glucose | 20 g |
| Trace elements | 1 ml |
| Agar | 20 g |
| dH2O | 1000 ml |
| *Mix well and autoclave at 121 °C for 15 min. pH 5.3 ± 0.2. | |
| Creatine sucrose agar (CREA,Frisvad 1981) | |
| Sucrose | 30 g |
| Creatine·1H2O | 3 g |
| K3PO4·7H2O | 1.6 g |
| MgSO4·7H2O | 0.5 g |
| KCl | 0.5 g |
| FeSO4·7H2O | 0.01 g |
| Trace elements stock solution | 1 ml |
| Bromocresol purple | 0.05 g |
| Agar | 20 g |
| dH2O | 1000 ml |
| *Mix well and autoclave at 121 °C for 15 min. pH 8.0 ± 0.2. | |
| Czapek's agar (CZ,Raper & Thom 1949) | |
| Czapek concentrate | 10 ml |
| Sucrose | 30 g |
| Trace elements stock solution | 1 ml |
| Agar | 20 g |
| dH2O | 1000 ml |
| *Mix well and autoclave at 121 °C for 15 min. | |
| Czapek Yeast Autolysate agar (CYA,Pitt 1979) | |
| Czapek concentrate | 10 ml |
| Sucrose | 30 g |
| Yeast extract (Difco) | 5 g |
| K2HPO4 | 1 g |
| Trace elements stock solution | 1 ml |
| Agar | 20 g |
| dH2O | 1000 ml |
| *Mix well and autoclave at 121 °C for 15 min. pH 6.2 ± 0.2. | |
| Czapek Yeast Autolysate agar with 5 % NaCl (CYAS) | |
| Czapek concentrate | 10 ml |
| Sucrose | 30 g |
| Yeast extract (Difco) | 5 g |
| K2HPO4K2HPO4 | 1 g |
| Trace elements stock solution | 1 ml |
| NaCl | 50 g |
| Agar | 20 g |
| dH2OdH2O | 1000 ml |
| *Mix well and autoclave at 121 °C for 15 min. pH 6.2 ± 0.2. | |
| Dichloran 18 % Glycerol agar (DG18,Hocking & Pitt 1980) | |
| Dichloran-Glycerol-agar-base (Oxoid) | 31.5 g |
| Glycerol (anhydrous) | 220 g |
| Trace elements stock solution | 1 ml |
| Chloramphenicol | 0.05 g |
| Agar | 20 g |
| dH2O | 1000 ml |
| *Mix well and autoclave at 121 °C for 15 min. After autoclaving, add 0.05 chlortetracycline. pH 5.6 ± 0.2. | |
| Malt Extract agar (MEA,Samson et al. 2010) | |
| Malt extract (Oxoid CM0059) | 50 g |
| Trace elements stock solution | 1 ml |
| dH2O | 1000 ml |
| *Mix well and autoclave at 115 °C for 10 min. pH 5.4 ± 0.2. | |
| Oatmeal agar (OA,Samson et al. 2010) | |
| Oatmeal / flakes | 30 g |
| Trace elements stock solution | 1 ml |
| Agar | 20 g |
| dH2O | 1000 ml |
| *First autoclave flakes (121 °C for 15 min) in 1000 ml dH2O. Squeeze mixture through cheese cloth and use flow through, topping up to 1000 ml with dH2O with 20 g agar. Autoclave at 121 °C for 15 min. pH 6.5 ± 0.2. | |
| Yeast extract sucrose agar (YES,Frisvad 1981) | |
| Yeast extract (Difco) | 20 g |
| Sucrose | 150 g |
| MgSO4·7H2O | 0.5 g |
| Trace elements stock solution | 1 ml |
| Agar | 20 g |
| dH2O | 885 ml |
| *Mix well and autoclave at 121 °C for 15 min. pH 6.5 ± 0.2. | |