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. 2014 Nov 28;46(11):e123. doi: 10.1038/emm.2014.73

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Copyright © 2014 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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miR-27 inhibits the mitochondrial fission factor (MFF) mRNA translation. (a) Polysome profiles. Cell lysates, prepared from CHANG liver cells transfected with pre-miR-27, anti-miR-27 or control miRNA, were fractionated through sucrose gradients. The arrow indicates the direction of sedimentation; − indicates fractions with no ribosomal components; 40S and 60S indicate small and large ribosomal subunits, respectively; 80S indicates monosomes; and LMWP and HMWP indicate low- and high-molecular-weight polysomes, respectively. (b) The relative distributions of the MFF (top) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs (bottom) were studied by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of each of the 13 fraction. The data are representative of two independent experiments.