Figure 5.

miR-27 increases mitochondrial activity. Mitochondrial membrane potentials (a) and mitochondrial ATP levels (b) after miR-27 regulation. (a) CHANG cells were transiently transfected with pre-miR-27, anti-miR-27 or control miRNA. Forty-eight hours after transfection, the cells were incubated with tetraethyl benzimidazoly carbocyanine iodide (JC-1) dye, and the relative mitochondrial membrane potentials were determined by measuring the fluorescence. (b) After transfection of pre-miR-27, anti-miR-27 or control miRNA, the cells were further incubated with galactose-containing medium, and the mitochondrial ATP levels were analyzed by measuring the luminescence after adding an ATP Detection Reagent. (c) The cell number was determined 48 h after transfection. The data represent the mean±s.e.m. from three independent experiments. *P<0.05 and **P<0.01 (d) Schematic diagram of the role of miR-27 in the regulation of mitochondrial dynamics in CHANG liver cells.