Figure 6. CXCR7 and CXCR4 Regulate Laminar Position of Transplanted MGE Cells.

(A) Schema depicting transplant of E13.5 CAG-dsRed⁺ MGE cells into P1 CXCL12-GFP reporter hosts.

(B–C′) Immunofluorescent images of transplanted MGE cells in the neocortex at 7 DPT, arrows in (C) and (C′) point to dsRed⁺ cells at the IV/V border that lie outside of the CXCL12-GFP⁺ domain in layer V. Brackets show the width of layer V. Scale bar in (C′) represents 250 μm.

(D) Quantification of CAG-dsRed⁺ cells in neocortical layers at 7 DPT.

(E) Schema depicting transplant of E13.5 Lhx6-GFP⁺ MGE cells into P1 WT hosts.

(F–H) Lhx6-GFP immunofluorescence (green) merged with DAPI (blue) in neocortex of mice in which either Control, CXCR4^−/− or CXCR7^−/− E13.5 Lhx6-GFP⁺ MGE cells were transplanted into P1 WT neocortex and assessed at 30 DPT. Scale bar in (H) represents 250 μm.

(I) Proportion of GFP⁺ Cells in Neocortical Layers of WT CXCR4^−/− and CXCR7^−/− MGE Cells

(J–Q) Immunofluorescence of 30 DPT transplants stained for SST (J–L) or PV (M–O), arrows point to coexpressing cells. Quantification of the proportion of Lhx6-GFP+ MGE cells that express SST (P) or PV (Q). Data are represented as mean ± SEM. Chi-square test (for lamination) or one-way ANOVA (for cell fate proportion) was used to test significance between groups. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar in (O) represents 100 μm.

See also Figure S7.