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Agarose gel electrophoresis of PCR products of Mper ERG11 amplified from genomic DNA. The reactions were done with an annealing temperature gradient. M - molecular weight marker (1 kb; Amresco). 1–5 -genomic PCR products with no region corresponding to the signal peptide (1,858 bp), 6–10 - genomic PCR products without the first transmembrane insertion (1,712 bp), 11–15 - genomic PCR products without the first and second transmembrane insertions (1,575 bp).
