Table 1. Percentage of Cells Positively-Stained for Intracellular ROS, Externalization of Phosphatidylserine, Caspase Activity, and Dead Cells Based on the in Vivo Staining of T. pseudonana Cells from Days 2 to 7 in Fe-Replete (+Fe) or Fe-Limited (−Fe) Conditions.
| time (D) | culture | ROS (%) | annexin (%) | caspases (%) | SYTOX (%) |
|---|---|---|---|---|---|
| 2 | +Fe | 4.1 | 2.6 | 6.5 | 11.9 |
| –Fe | 5.5 | 8.6 | 10.2 | 13.2 | |
| 3 | +Fe | 5.1 | 4.8 | 7.0 | 10.3 |
| –Fe | 6.6 | 5.7 | 20.0 | 13.0 | |
| 4 | +Fe | 5.7 | 5.1 | 9.6 | 26.6 |
| –Fe | 24.4 | 16.0 | 25.4 | 22.5 | |
| 5 | +Fe | 18.8 | 15.5 | 35.5 | 28.1 |
| –Fe | 40.4 | 28.0 | 47.8 | 31.7 | |
| 6 | +Fe | 13.8 | 12.6 | 39.2 | 30.7 |
| –Fe | 49.3 | 31.1 | 65.2 | 32.7 | |
| 7 | +Fe | 24.0 | 18.6 | 55.7 | 29.5 |
| –Fe | 31.9 | 49.0 | 61.6 | 49.9 |