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Graphical representation of the cumulative number of cultured species from Bacteria, Archaea and Eukarya from the human gastrointestinal tract as a function of time. The arrows indicate the turning points of the gastrointestinal microbiota research: (1) Isolation of the first gastrointestinal bacterial species, (2) Introduction of strictly anaerobic techniques, and (3) Introduction of molecular techniques in the field of the gastrointestinal microbiota research.
