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. 2014 Dec 5;14:135. doi: 10.1186/s12935-014-0135-3

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© Duan et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PI3K-AKT signaling is required for M. hyorhinis infection and induced cell migration in MGC803 cells. (A) AG1478 or wortmannin pretreatment inhibits M. hyorhinis infection. AG1478 or wortmannin was added in cell medium 1 hour prior to M. hyorhinis exposure. Then the cells were subjected to PCR amplification of p37. (B) and (C) AG1478 or wortmannin abolishes M. hyorhinis (B) and GST-p37 (C) induced cell migration. Transwell cell migration assay was performed according to manufacturer’s protocol. Values represented the mean ± SD from three to four independent experiments with triplicate samples. *, P <0.05.