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. 2014 Sep 12;8(6):628–635. doi: 10.1111/irv.12285

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© 2014 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Hemagglutination inhibition (HI) and microneutralization (MN) titers and avidity ELISA for mice with HI titers between 80 and 160. Serum samples with HI titers between 80 and 160 were selected (n = 6 mice per group). (A) geometric mean titer (GMT) HI and MN titers for each group. (B) Avidity ELISAs were performed with the addition of 5-min incubation with either PBST or 4M urea. Values are shown as GMT. Differences between PBS and urea-treated samples are indicated by ‘†’ (P < 0·05). (C) The avidity index (AI) was calculated for each sample. Values are shown as average AI (percent). Error bars represent 95% confidence intervals. Differences between groups are indicated by * (0·0001 < P < 0·05) or ** (P < 0·0001).