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Reverse-transcriptase polymerase chain reaction (RT-PCR) analysis showing expression patterns of the pathogenesis-related gene 1 (VfPR1) and gene 2 (VfPR2) in the leaf samples of resistant (Nubaria) and susceptible (Giza 40) FB cultivars at different time points after inoculation with B. fabae as compared with un-inoculated controls. The elongation factor 1-alpha (VfELF1A) gene was used as a reference gene. The experiment was conducted twice with similar results.
