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First and second selections of primer sets for the IYSV detection. Panel A. First selection of specific primer sets. Lane M, 100 bp step DNA Ladder maker (Genepia, Korea); lanes 1–24, IYSV-specific primer sets 1–24; dot, selected primer set. Panel B. Second selection of non-specific primer sets. lane number, primer set number; TSWV, Tomato spotted wilt virus; TRV, Tobacco rattle virus.
