Table 1.

Selected primer sets for RT-PCR and nested PCR of IYSV

Primer			Sequence (5′→3′)	Length (nt)	Product (nt)
Set	PCR	Name
1	RT	IYS_N10	TCTATCTTTCTTGGAGGGAG	20	249
		IYS_C60	TCAACTTACGAGCAACTCTG	20
	Nested	IYS_N20	ATCAATGAAGCAGCACCC	18	146
		IYS_C60	TCAACTTACGAGCAACTCTG	20
12	RT	IYS_N30	GCTCGTAAGTTGAGAATCTGC	21	307
		IYS_C40	TGGACATTCAGGAGGTTG	18
	Nested	IYS_N40	TGCCATCAAAGTGAGAGG	18	210
		IYS_C40	TGGACATTCAGGAGGTTG	18