Table 2.
Description of samples positive by PCR, Nested PCR and Differential PCR
| S. no. | Sample | PCR | NPCR | Differential PCR | Age (months) | Sex | Breed | Vaccination status | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2 | 2a | 2b | 2c | Either of 2, 2a, 2b or 2c | ||||||||
| 1. | P1 | − | + | + | + | − | − | + | 4 | F | GSD | + |
| 2. | P2 | − | + | − | − | − | − | − | 3.5 | M | St. Bernard | + |
| 3. | P5 | − | + | − | − | − | − | − | 1.5 | M | ND | + |
| 4. | P9 | − | + | − | − | − | − | − | 5 | M | ND | + |
| 5. | P10 | − | + | − | + | + | − | + | 2 | F | Labrador | + |
| 6. | P12 | − | + | − | + | − | − | + | 2 | M | Labrador | + |
| 7. | P13 | − | + | − | − | − | − | − | 1.5 | M | Rottweiler | + |
| 8. | P14 | − | + | − | − | − | − | − | 2.5 | M | GSD | + |
| 9. | P15 | − | + | − | + | − | − | + | 3 | F | Cocker spaniel | + |
| 10. | P16 | − | − | − | + | + | − | + | 3 | M | Rottweiler | − |
| 11. | P17 | − | − | − | + | − | − | + | 1 | F | St.Bernard | − |
| 12. | P18 | − | + | − | − | − | − | − | 3.5 | F | Labrador | − |
| 13. | P19 | − | + | − | − | − | − | − | 4.5 | M | Rottweiler | + |
| 14. | P20 | − | + | − | − | − | − | − | 3 | M | Labrador | + |
| 15. | P21 | − | + | − | − | − | − | − | 4 | M | Pom | + |
| 16. | P22 | − | − | − | + | − | − | + | 1.5 | F | Labrador | − |
| 17. | P23 | − | − | − | + | + | + | + | 2.5 | M | GSD | + |
| 18. | P26 | − | + | − | − | − | − | − | 2.5 | M | ND | − |
| 19. | P28 | − | + | − | − | − | − | − | 2 | M | Rottweiler | + |
| 20. | P29 | − | + | − | − | − | − | − | 2 | M | ND | − |
| 21. | P30 | − | − | − | − | + | − | + | 1 | F | Rottweiler | − |
| 22. | P31 | − | + | − | − | − | − | − | 1.5 | M | Rottweiler | − |
| 23. | P32 | + | + | − | + | + | + | + | 2 | F | Labrador | + |
| 24. | P34 | + | + | + | + | + | + | + | 4.5 | M | ND | − |
| 25. | P35 | + | + | + | + | + | + | + | 3 | F | Dalmatian | − |
| 26. | P36 | − | + | − | − | − | − | 2 | M | Pug | + | |
| 27. | P37 | − | − | − | − | + | − | + | 5 | M | ND | − |
| 28. | P39 | − | + | + | + | + | + | + | 1.5 | M | Labrador | − |
| 29. | P40 | − | − | − | − | + | − | + | 2.5 | F | Labrador | − |
| 30. | P41 | − | + | − | + | + | − | + | 5 | F | St.Bernard | − |
| 31. | P42 | − | − | − | + | + | − | + | 3 | M | Labrador | + |
| 32. | P43 | − | + | − | + | + | + | + | 4.5 | F | Labrador | − |
| 33. | P44 | − | + | − | − | − | − | − | 6 | F | Labrador | + |
| 34. | P45 | + | + | + | − | + | + | + | 3 | M | Labrador | − |
| 35. | P46 | + | + | + | + | + | + | + | 1.5 | M | ND | − |
| 36. | P47 | + | + | + | + | + | + | + | 3 | F | St.Bernard | − |
| 37. | P48 | − | − | + | − | + | + | + | 2 | F | Labrador | − |
| 38. | P49 | + | + | + | + | + | + | + | 3 | F | Labrador | − |
| 39. | P50 | − | + | + | + | + | + | + | 6 | F | Labrador | + |
| 40. | P56 | − | + | − | − | − | − | − | 8 | F | Pug | + |
| 41. | P61 | − | + | − | − | − | − | − | 10 | M | Mongrel | − |
| 42. | P64 | − | − | − | + | − | + | + | 18 | M | GSD | + |
| 43. | P65 | − | − | − | − | − | + | + | 4.5 | M | Dash hound | + |
| 44. | P68 | − | − | − | + | − | − | + | 2.5 | M | Rottweiler | + |
| 45. | P69 | − | − | − | + | − | + | + | 3 | M | Labrador | + |
| 46. | P71 | − | − | − | − | + | + | + | 2 | F | Sharpie | − |
| 47. | P72 | − | − | − | − | + | + | + | 2.5 | F | GSD | − |
| 48. | P73 | − | − | − | + | + | − | + | 3 | M | Dash hound | − |
| 49. | P74 | − | − | + | + | + | + | + | 6 | M | GSD | − |
| 50. | P75 | − | + | + | − | + | + | + | 10 | M | GSD | − |
| 51. | P76 | − | − | + | + | − | + | + | 4 | M | ND | − |
| 52. | P79 | − | + | + | − | − | + | + | 4 | M | GSD | − |
| 53. | P80 | − | + | − | − | − | − | − | 6 | M | GSD | − |
| 54. | P81 | − | − | − | + | − | − | + | 2 | M | Labrador | − |
| 55. | P82 | − | + | + | + | − | + | + | 5 | F | GSD | − |
| 56. | P83 | − | + | + | + | − | + | + | 3 | M | ND | − |
| 57. | P84 | − | + | − | + | − | − | + | 1.5 | M | ND | − |
| 58. | P85 | − | + | + | + | − | − | + | 1 | F | Pom | − |
| Total | 7 | 39 | 17 | 30 | 24 | 23 | 40 | |||||
(−) Negative/(+) Positive by PCR, Nested PCR and Differential PCR; M Male; F Female; GSD German shepherd dog; ND Non descript; Pom Pomeranian