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A, cytoplasmic [cAMP] in 3T3-L1 adipocytes incubated with forskolin (10 μm) + IBMX (200 μm) for 30 min in the presence or absence (pre-treatment with 50 μm BAPTA-AM) of intracellular Ca2+. B, example trace of effect of forsk–IBMX on 3T3-L1 adipocyte [Ca2+]i. **P < 0.01; ***P < 0.001.
