Fig. 6.
Changes in the cell density of R. erythropolis PR4 transformants in the n-hexadecane phase as a function of the vortexing time in a two phase system consisting of n-hexadecane and PP13 TFSI (N-methyl-N-propylpiperidinium bis (trifluoromethanesulfonyl) imide, with a viscosity of 150 mPas at 25°C; electric conductivity, 1.5 mS cm−1; melting point, 12°C; water content, <50 ppm). Cells were grown in IB medium containing C12 and then applied to this assay. ●, pK4-EL2-1; ○, pK4-ΔEL2-1. A0 and At indicate the optical densities in the alkane phase before and after mixing. Each value is the average of at least 3 replicates from at least 3 independent experiments. Standard deviations are presented in error bars. Striate and dashed lines indicate the initial removal rates (R0) of pK4-EL2-1 and pK4-ΔEL2-1, respectively.