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. 2014 Sep 20;29(4):353–362. doi: 10.1264/jsme2.ME14052

Fig. 5.

Fig. 5

Neighbor-joining distance matrix tree showing phylogenetic positions of the 16S rRNA gene clones obtained from ANSBR 1 on d 91. Representative clones of the 35 OTUs are shown with their closest relatives of established species and/or uncultured bacteria whose sequences were retrieved from the public database. The sequence of Aquifex pyrophilus (M83548) was used as the outgroup to root the tree. The nodes supported by a bootstrap value of more than 80% are shown by closed circles. Bar = 2% nucleotide substitution rate (Knuc).