Table 2.
16S rRNA gene copies and statistics of detected sequences
| Unincubated | 40°C | 50°C | 60°C | 70°C | 80°C | |
|---|---|---|---|---|---|---|
| Bacteria/archaea | ||||||
| 16S rRNA gene abundances g−1 of slurry1 | 1.2×105/6.0×104 | 1.3×105/1.3×103 | 3.3×104/1.6×103 | 2.0×103/BDL | BDL/BDL | NA/NA |
| Number of clones | 54/45 | 42/56 | 56/58 | 59/25 | 56/41 | 36/35 |
| Number of ribotypes | 33/5 | 14/2 | 10/5 | 12/2 | 7/1 | 3/1 |
| Number of bacterial (sub)phyla/archaeal orders | 14/4 | 8/2 | 5/3 | 7/2 | 4/1 | 3/1 |
| Shannon-Wiener index | 3.31/1.30 | 2.42/0.89 | 1.59/0.53 | 1.74/0.67 | 1.15/0 | 1.02/0 |
| Chao1 index | 69.8/4.0 | 26.5/2.0 | 20.0/5.0 | 22.5/2.0 | 7.3/1.0 | 3.0/1.0 |
| Coverage (%) | 61/100 | 88/98 | 91/98 | 88/100 | 98/100 | 100/100 |
1
Slurry refers to unincubated or incubated samples. Bacterial 16S rRNA gene abundances were obtained on the basis of duplicate analyses. Uncertainties were within 10% of the median value. However, archaeal 16S rRNA gene abundances were obtained from single analyses due to limited amounts of genomic DNA.
BDL: Below detection limit. NA: Not available due to insufficient genomic DNA for qPCR analysis.