Fig. 2.

Biofilm formation by Methylobacterium strains on 96-well polystyrene plates. The overnight cultures of Methylobacterium strains were diluted and transferred to each well of a 96-well polypropylene microtiter dish. After being incubated at 30°C for 48 h, biofilms were stained with crystal violet and estimated by analyzing absorbance at 595 nm. Six wells of each sample were used to measure biofilm formation, and error bars indicate standard deviations. Single biofilms (black bars), mixed biofilms with P-1M (white bars), and mixed biofilms with P-18S (grey bars) are shown.