Fig. 2.

Quantification of CVB4 RNA and level of infectious particles. 4 μL of culture supernatant fluid containing CVB4 were applied on aluminium foil in quadruplicate. Inocula on aluminium foil were treated 15 s at 110°C, and thereafter recovered. The infectious titers of inocula were determined and expressed as log₁₀ TCID₅₀ 4 μL⁻¹ (■). RNA was extracted from the harvest fluid and the level of viral RNA was measured by quantitative RT-PCR and expressed as C_t (□). The results are the mean + SD of four independent experiments.