Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference (s) |
|---|---|---|
| Pseudomonas fluorescens | ||
| Pf0-1 | wild-type strain | (8) |
| Pf01Rif | Pf0-1 derivative, spontaneous rifampicin-resistant mutant | (25) |
| FLD3 | Pf0-1 derivative, ΔctaA (Pfl01_4431) ΔctaB (Pfl01_0124) ΔctaC (Pfl01_0354) | (25) |
| FLD3Rif | FLD3 derivative, spontaneous rifampicin-resistant mutant, ΔctaA ΔctaB ΔctaC | (25) |
| KPF09 | Pf0-1 derivative, ΔmcpS (Pfl01_0728) | This study |
| KPF21 | Pf0-1 derivative, ΔmcpT (Pfl01_3768) | This study |
| OX1 | Pf0-1 derivative, ΔmcpS ΔmcpT | This study |
| FLD5 | Pfl0-1 derivative, ΔctaA ΔctaB ΔctaC ΔmcpS ΔmcpT | This study |
| FLD5Rif | FLD5 derivative, spontaneous rifampicin-resistant mutant, ΔctaA ΔctaB ΔctaC ΔmcpS ΔmcpT | This study |
| Pf0-1 ΔcheA | Pf0-1 derivative, ΔcheA (Pfl01_1566) | (25) |
| Pseudomonas aeruginosa | ||
| PAO1 | wild-type strain | (12) |
| RPc | PAO1 derivative, PA2652 gene knockout mutant | This study |
| Escherichia coli | ||
| JM109 | recA1, endA1, gyrA96, thi-1, hsdR17 (rk− mk+), e14− (mcrA−), supE44, relA1, Δ (lac-proAB)/F′[traD36, proAB+, lacIq, lacZ ΔM15] | (27) |
| S17-1 | MM294, RP4-2 Tc::Mu-Km::Tn7 chromosomally integrated | (31) |
| Plasmids | ||
| pUCP18 | Escherichia-Pseudomonas shuttle vector; Cbr | (29) |
| pFLCP09 | pUCP18 with a 2.5 kb PCR fragment containing mcpS (Pfl01_0728); Cbr | This study |
| pFLCP21 | pUCP18 with a 2.1 kb PCR fragment containing mcpT (Pfl01_3768); Cbr | This study |
| pK18mobsacB | Kmr pUCP18 derivative, lacZα, mob site, sacB | (28) |
| pNMFL09 | pK18mobsacB with a 1.1-kb PCR fragment upstream of mcpS and 0.9-kb PCR fragment downstream of mcpS; Kmr | This study |
| pNMFL21 | pK18mobsacB with a 1.5-kb PCR fragment upstream of mcpT and 1.2-kb PCR fragment downstream of mcpT; Kmr | This study |
| pNMPAR | pK18mobsacB with a 1.6-kb PCR fragment upstream of the PA2652 gene and 1.2-kb PCR fragment downstream of the PA2652 gene; Kmr | This study |
Cbr, carbenicillin resistance; Kmr, kanamycin resistance.