Figure 9. The intrinsic enthalpy – entropy compensation graphs for the 16 studied compound binding to five CA isoforms.

Diagonal central line represents the dissociation constant of 1.0 nM (Δ_bG = −53.4 kJ/mol at 37°C) while adjacent dashed lines show extremely tight binding of 10 pM or lower affinity at 100 nM. The compounds exhibit the range of affinities between 0.01 and 10 nM (Δ_bG between −65.3 and −47.5 kJ/mol) while the enthalpies varied to a greater extent between −14 and −68 kJ/mol. The entropies (TΔ_bS) also span the wide range between 11 and −41 kJ/mol.