Table 2. CD data of the peptides.
Peptidesa | PBS | SDS | TFE | |||
[θ]222 b | % helixc | [θ]222 | % helix | [θ]222 | % helix | |
RI16 | −113 | 1 | −1670 | 11 | −11784 | 78 |
T9W | −212 | 1 | −6010 | 40 | −12369 | 82 |
T9I | −728 | 5 | −5849 | 39 | −9073 | 60 |
T9K | −337 | 2 | −1960 | 13 | −8986 | 59 |
T9F | −533 | 4 | −9443 | 62 | −15123 | 100 |
The peptides (150 µM) were dissolved in 10 mM PBS (pH 7.4), 50% TFE, or 30 mM SDS.
The mean residue molar ellipticities [θ]222 (degree•cm2•dmol−1) at wavelength 222 nm were measured at 25°C.
The helical content (%) of a peptide relative to the molar ellipticity value of peptide T9F in 50% TFE.