Table 3. Antimicrobial activity and cytotoxic activity of parental and mutant peptides.

Items	MICa (µM)						MBCa (µM)
	RI16	T9W	T9I	T9K	T9F	Amikacin	T9W
E. coli 25922	128	64	128	128	64	0.125	>128
S. typhimurium 7731	128	128	256	256	128	0.5	>128
S. aureus 29213	128	128	256	128	128	1	>128
S. epidermidis 12228	32	64	32	128	32	0.125	>128
P. auruginosa 27853	256	2	256	128	256	1	2
P. auruginosa 10419	256	4	256	256	256	2	4
P. auruginosa 21625	256	4	256	256	256	16	4
P. auruginosa 21630	256	4	256	256	256	1	4
P. auruginosa LC	32	1	64	256	64	0.25	2
P. auruginosa LCCIb	256	2	256	256	256	8	4
P. auruginosa LCGEc	256	4	256	256	256	>1280	8
P. auruginosa LCCEd	256	4	256	256	256	8	8
Hemocytes (HC50 e)	>256	>256	>256	>256	>256	>256	>256
Macrophage (LD50 f)	>256	>256	>256	>256	>256	>256	>256

^aMinimal inhibitory concentration (MIC) was determined as the lowest concentration of the peptides that inhibited bacteria growth, and minimal bactericidal concentration (MBC) was the concentration resulting in no bacterial growth.

^bA ciprofloxacin-resistant strain, which MIC for ciprofloxacin was above 512 µM.

^cA gentamincin-resistant strain, which MIC for gentamincin was above 512 µM.

^dA ceftazidime-resistant strain, which MIC for ceftazidime was above 512 µM.

^eHC₅₀ value was taken as the concentration of peptide producing 50% hemolysis.

^fLD₅₀ value was taken as the concentration of peptide producing 50% cell death.