Table 1. Pairs of primers used in the study.
Cell cycle control genes | ||
SMAD2 | F | 5′AGGGGAGAGGAGGAAGAAGG3′ |
SMAD2 | R | 5′CGGAGGAGTGAATGGCAATA3′ |
CDK7 | F | 5′GTGCAGCTGGTGATGATCTG3′ |
CDK7 | R | 5′GCAGCTGATTTCCTGGAGTG3′ |
CCND1 | F | 5′CTCCTACTTCAAGTGCGTGC3′ |
CCND1 | R | 5′ACTTCCTCTTCGCACTTCTG3′ |
PRKAA1 | F | 5′CGGTGGCGGATAAACAGAAG3′ |
PRKAA1 | R | 5′TCAGGATCTTCACGGCAACT3′ |
E2F6 | F | 5′TCAGCCATGGAAGAAGCTCT3′ |
E2F6 | R | 5′TCCTGAAATGCCTGAATGCT3′ |
MAPK12 | F | 5′GGCAAGGCATACAGACAGTG3′ |
MAPK12 | R | 5′TACAGCCCACAGACCAGATG3′ |
CDK1 | F | 5′CAGCCTCGAAACACATGTCA3′ |
CDK1 | R | 5′GCAGGAAGAGTGGATTTGTCC3′ |
P21 | F | 5′CTGCCTCCCAATCCAGAAGA3′ |
P21 | R | 5′AGTCTGAAGGGAGGGAGATCT3′ |
Growth hormone pathway genes | ||
GHR | F | 5′ATCCACCACCAACAGCAGAT3′ |
GHR | R | 5′ACCATTGTTGAGAGCCTGG3′ |
GH | F | 5′GTCAAGCAACACCTGAGCAA3′ |
GH | R | 5′ACAGCGATGAGGAGAGGAGA3′ |
JAK2 JAK2 | FR | 5′CCTAAAGAGTAACAAGAAGCTGC3′5′TCCACGGATGTCACCATTCT3′ |
Cell energy metabolism genes | ||
H6PD | F | 5′GAGTTTCCAGCTGCTTTTGC3′ |
H6PD | R | 5′CTCAGCATCCTGGGAGAGAG3′ |
FBP1 | F | 5′ACAAAGATGCTGTGATAGTGGA3′ |
FBP1 | R | 5′CAGAAGGTTCATTAGGGGACAC3′ |
Stress response genes | ||
NR3C1 | F | 5′TGGAATAGGTGCCAGGGATC3′ |
NR3C1 | R | 5′GGTTGTGGATGGAGAAGAGC3′ |
NR3C2 | F | 5′TCATTCGGAGTTGGCATCCA3′ |
NR3C2 | R | 5′TCTCCACACACCAAACACAC3′ |
Steroidogenic gene | ||
NR5A1 | F | 5′CACGGAGCTGCATCTTCTTC3′ |
NR5A1 | R | 5′AGCTGTCGGTAGATGTGGTC3′ |
Housekeeping genes | ||
β-actin | F | 5′CTGGCACCACTCCTTCTACA3′ |
β-actin | R | 5′ATACATGGCTGGGGTGTTGA3′ |
GAPDH | F | 5′CGAAGCGGTAAAGATGGTGA3′ |
GAPDH | R | 5′ATGAAGGGATCGTTGATGGC3′ |
Sex chromosome specific primers | ||
W1 | F | 5′GGGTTTTGACTGACTAACTGATT3′ |
W2 | R | 5′GTTCAAAGCTACATGAATAAACA3′ |
Z1 | F | 5′GTGTAGTCCGCTGCTTTTGG3′ |
Z2 | R | 5′GTTCGTGGTCTTCCACGTTT3′ |