Table 1. NADPH-dependent 3-hydroxyacyl-CoA dehydrogenase activity in WT and Δtfeα1/Δtfeα1 cells.
| 3 hydroxyacyl-CoA | GPDH3 | |
| dehydrogenase3 | ||
| WT WCE1+gluc4 | 6.62±0.63 (n = 5) | 32.20±3.48 (n = 3) |
| WT WCE1−gluc5 | 5.22±0.40 (n = 5) | 34.92±2.71 (n = 3) |
| Δtfeα1/Δtfeα1 WCE1+gluc4 | 6.04±0.71 (n = 5) | 22.80±2.45 (n = 3) |
| Δtfeα1/Δtfeα1 WCE1−gluc5 | 5.00±0.47 (n = 5) | 35.40±1.89 (n = 3) |
| WT glyco2+gluc4 | 11.76±0.52 (n = 6) | 213.18±4.12 (n = 3) |
| Δtfeα1/Δtfeα1 glyco2+gluc4 | 9.12±0.77 (n = 6) | 208.22±12.19 (n = 3) |
1
WCE, whole cell exctract.
2
glyco, partially purified glycosome fraction.
3
Mean ± SEM of n experiments (mU/mg of protein).
4
+gluc: cells cultured in SDM79 containing 10 mM glucose.
5
−gluc: cells cultured in glucose-depleted SDM79GluFree.